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Complete chapter 5

Complete the game with no life upgrade

Complete the game with both difficulties set to hard

Earn all gold medals in story mode

Earn all diamond medals in story mode

Complete sample 8 without destroying a single bloated cell

Complete sample 12 without touching a single sticky web

Complete sample 14 without slowing the chain

Complete sample 17 without being poisoned once by toxins

Complete sample 21 without colliding with a single flagellate pathogen

Be awarded with an extra chain reaction in chapter 4 or 5

Reach the last wave difficulty in all challenges